Suspected first type H1N1 must make a definite diagnosis of four over barriers

Suspected first type H1N1 must make a definite diagnosis of four over barriers
Suspected first type H1N1 must make a definite diagnosis of four over barriers
Author: Shen Wang, speech of Ming, Wei, to suspected first type patient, influenza of H1N1, through which kind of could means make a definite diagnosis of their, infect first type H1N1 influenza virus finally? In the afternoon of yesterday, Xuanwu District of Beijing saw the New Year in in the street No. 100, reporters visited China's disease and accused of the national influenza central lab of virosis of centre on the spot, how from the basic passage obtaining the sample and isolating the virus in the suspected patient's respiratory tract staff members have reproduced!, and then through reading the gene sequence of the virus under the special instrument, contrast with the gene sequence picture of first type H1N1 influenza virus finally, thus obtain the whole course whether a patient catches epidemic disease. A delivery transports the virus sample at constant temperature of 4 ℃ Place: The hospital is used in demanding the respiratory tract from suspected patient of sample of the virus the network laboratory of influenza, it is generally liquid, namely throat liquid or nose liquid. Each liquid sample demand is more than 200 micrograms. According to the introduction of national influenza Li XiaoDan, staff member of laboratory, there is only enough sample amount, it could guarantee to separate sufficently to viruses living and using for nuclear for acid virus genetic fragment that be measured. In order to guarantee the activity of the virus, each sample is after the patient's nose or swallowing department is taken out, must put into leak protection, explosion-proof, rain -proof special airtight container immediately. Li XiaoDan says, in the container, should guarantee 4 ℃ constant temperature, " just like the cool room of the refrigerator " ,Been insisting on from hospital to the laboratory all the time. Later, were sent to P3 laboratory (the living beings protected the tertiary laboratory) of the very heavy organ by the special messenger . 2 drew the virus and packaged the genetic fragment Place: The living beings protect the tertiary experiment room P3 laboratory and once show up prominently in the fight of resisting SARS and bird flu more than once. Prevent and control influenza and prevail big, find the trace of first type H1N1 influenza virus, the work in P3 laboratory in time, it is still foundation and key. The staff members who only obtained the particular qualification, could open the password to lock, enter P3 laboratory. Staff members need to cross a locker room, two locker rooms, buffer one and prepare 4 rooms sequentially, arm to the foot from the beginning finally. Holding and putting on the actor's headgear, segregation clothes connected with the sweater and pants, after gauze mask and binding, could enter the laboratory to begin to work. With deepen, atmospheric pressure lower, come down from level to level too, atmospheric pressure on the laboratory have only 50-60 about and (much lower than normal atmospheric pressure) handkerchief . Only in this way, could guarantee wind of inside will blow outside, microorganism and virus inside, will not go to the outdoor experiment. According to the introduction of Li XiaoDan, measure the work of first type H1N1 influenza virus, will launch from two respects at the same time: A part of the sample is used for drawing the virus alive, wait until and train studying. Another part, is used for isolating the sour liquid of core that the virus hit, only " fill with the passage of the gene code " ,Needed more than 50 micrograms, fen prepared against further detection after packing into the test tube. 3 is measured Expand the genetic code and facilitate the contrast Place: The living beings protect second laboratory (pcr lab) The genetic fragment in the test tube will be sent to the pcr lab subsequently. Li XiaoDan says, PCR appearance is that the nuclear acid measures the key equipment of the virus. In the last few days, detection reagent box which they developed to first type H1N1 influenza, will play a main role in this link. Experiment personnel put a group of genetic fragments in the test tube into measuring in the response liquid that reagent disposes, put under PCR appearance again, about about two and a half hours, each genetic fragment is enlarged, turn into the nuclear acid not of uniform size and expand the thing CDNA ' The genetic fragment expands things) . At the same time, another part is through reflecting liquids, the core impractical RNA (ribonucleic acid) packaged in PCR little test tube ,Can be in the purpose-built template, the ones that passed with virus genetic fragment of goal are at regular time and quantity than correctly, experiment personnel read the result: Whether it is first type H1N1 influenza virus. XiaoDan Li say for guarantee, find to have error accurately result, this piece PCR finds the result at regular time and quantity, one demanding the conclusion only as the whole of first type H1N1 influenza virus is consulted. 4 is made a definite diagnosis of Sift and show the virus in the glue of electric swimming " The true features " Place: The living beings protect the first class laboratory ' Comprehensive laboratory) The small test tube containing different genetic fragments and expanding the thing is sent to the comprehensive laboratory, the key equipment here is that have filled candy gel of agar, rectangle electric swimming detector that is inserted positive negative electrode of both ends with. Utilize the liquid moving device, after experiment personnel dye the micro- colourless transparent liquid in the test tube, and then put the glue charged " The pool " . Until voltage draw, promote, glue surface of pool begin, rise little bubble while being intensive, "that genetic expansion thing that is virus walks toward another one, the product with big stature walks slowly, the faster the product the with small more stature walks. " Li XiaoDan says, the electric swimming appearance of candy gel of agar is like a sieve, each genetic fragment in a pair of virus is with the particular " big " , it is " small " The image displays. Certainly, the above-mentioned scene people's naked eye is unable to see. Experiment personnel let " The glue pool " Charge for 40 minutes, guarantee all expansion thing " sports " After standing up, " glue pool " Put into another one purpose-builtly and squarely " The iron cupboard " China, this is actually " camera " working under an ultraviolet ray ---Appearance of the formation of image of gel. In the appearance of the formation of image, the ultraviolet lamp gives out light, shine on the gel, then to dyeing the expansion thing giving out light to take a picture in the glue automatically, and export on the computer screen that is connected. Li XiaoDan introduces, can also access the first type H1N1 influenza virus gene sequence picture in the computer at the same time, on the same position, are expanded the thing size and object of reference to totally coincide by several genetic fragments which examines the virus, can judge finally, the sample is inspected to a patient by ready samples and has been infected with first type H1N1 influenza virus. On the contrary, it is possible to exclude from infecting. Li XiaoDan says, sends to the laboratory and gets to detect the result finally from the virus sample, it nearly takes 4- 6 hours. The first type H1N1 virus measures a procedure doctor to take a sample to the suspected patient's nose liquid or throat liquid Send into the network laboratory of influenza and draw a plurality of genetic fragments, expand (enlarge) the genetic fragment one by one Expand things and enter the glue to dye, give out light to form images under the ultraviolet lamp In contrast to virus genetic fragment of goal stored in the computer, on the same position, it is unanimous whether the size is totally unanimous can judge finally, the sample is inspected to a patient by ready samples and has been infected with first type H1N1 influenza virus It solicit contributions piece originally it interview and write / solicit contributions originally staff reporter speech Wei piece photograph /staff reporter Shen Wang


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