The third generation of gene order-checking appearance comes out Can detect DNA chemistry to change

The third generation of gene order-checking appearance comes out Can detect DNA chemistry to change
The third generation of gene order-checking appearance comes out Can detect DNA chemistry to change
According to February 8 report of Nature website, " genome biology and technological progress conference " held on Ma Ke island in the state in Florida of U.S.A. last weekend Go, the biological scientific technical corporation of Pacific Ocean from California has introduced the third generation of gene order-checking appearance developed of it, it's time to check order the appearance has realized single member of a molecular formula of mark reads once rapidly. Researchers point out, the key advantage of the third generation of appearance of checking order can be to single DNA (DNA) The molecule checks order, and the mainstream on the market checks order the appearance can only be averaged molecule colony and checked order at present. The single member can make a variation to analyze to the rare array in DNA, do not need to enlarge DNA sample before checking order either to check order, because enlarging the course may initiate the mistake, cause and measure and fail to a certain DNA array. Its operation principle is to limit the duplication of DNA in a small interval with a kind of polymerase, giving various bases in addition, fluoro shows the track mark, when the base formate DNA chain, these fluorogenic marks will send out the flash of light of different colors, can recognise different bases according to flashing color. User's operation report shows, it is 1500 pairs that the new instrument reads the right average length of base, this is representing the his luminal company (Illumina) of the technological development level at present of this field 10 times of appearance of checking order produced. It is the longer to read the length, it is the easier to splice DNA array passage into an intact genome array. Last December, the triumphant morality and research group of Sri Lankan of Eric, chief scientific officer of company followed the trail of the origin of Haitian cholera with these new instruments. They have carried on gene order-checking to 5 S-shaped cholera bacteria, has finished all order-checking tasks in less than one hour, and check order with 150 bases of his luminal the appearance need one week. Pacific Ocean biological scientific technical corporation propose, realize in 15 minutes finishing the the whole gene order-checking of man by 2013 in 2008, and this job took one month at that time. The medical college of Texas Houston Beile checks order thatch subject of technologist Michael's wheat expresses, the single member checks order the appearance represents the future of DNA sequence, but the maximum obstacle of this technology is that fault rate is high at present. Other rate of accuracy of the appearance of checking orders can be more than 99%, and according to the operation report, the rate of accuracy of instrument of the biological scientific technical corporation of Pacific Ocean is about 85%. But triumphant Germany of Sri Lankan thinks, this shortcoming can be overcome by checking order repeatedly. Researchers claim, this instrument is expected to enter the market in the second quarter of this year, 700,000 dollars for each cost, it will be lower by 125,000 dollars than the appearance of checking order newly of his luminal company, though is unlikely to cause assaulting to the market in short term, some chemistry that it can measure DNA changes, will exhibit one's skill in field that therefore it is difficult to work in for instance, appearance of checking order of the tradition at present of waiting of epigenetics. (often beautiful monarch)


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